Journal: Journal of Applied Genetics

Article Title: Searching for new molecular markers for cells obtained from abdominal aortic aneurysm

doi: 10.1007/s13353-021-00641-4

Figure Lengend Snippet: Summary of the layers and patients

Article Snippet: The patients’ EL of AAA fragments and control cells, for them Human Aortic Adventitial Fibroblasts (AoAF) (Lonza, Basel, Switzerland) were cultured in SCGM Stromal Cell BulletKit (Lonza, Basel, Switzerland).

Techniques:

Journal: Journal of Immunology Research

Article Title: Distinct Redox Signalling following Macrophage Activation Influences Profibrotic Activity

doi: 10.1155/2019/1278301

Figure Lengend Snippet: Effect of macrophage coculture on collagen and alpha-smooth muscle actin expression in human aortic adventitial fibroblasts. PDBu-differentiated THP-1 macrophages were left untreated (M Φ ) or activated with IFN- γ +LPS or IL-4 for 72 hours in cell culture inserts. THP-1 macrophages were subsequently transferred to wells containing aortic adventitial fibroblasts, stimulated with 10 μ M PDBu in the absence or presence of 1000 U/ml PEG-catalase, and incubated for 24 hours. (a) Collagen 1 and (b) α SMA protein were measured in aortic adventitial fibroblasts, n = 10‐12. (c) Representative blot, depicting n = 1, is shown with GAPDH used as a loading control. Results presented as mean ± SEM, ∗ P < 0.05, ∗∗∗ P < 0.001 (1-way ANOVA followed by Sidak's post hoc test).

Article Snippet: Primary human aortic adventitial fibroblasts (AoAF; Lonza no. CC-7014; Lonza) were grown in Stromal Cell Growth Medium (SCGM; Lonza no. CC-3205), containing 5% FBS and used from passages 2 to 8.

Techniques: Expressing, Cell Culture, Incubation, Control

Journal: Journal of Biomedical Materials Research. Part a

Article Title: Differential effects of substrate modulus on human vascular endothelial, smooth muscle, and fibroblastic cells

doi: 10.1002/jbm.a.34075

Figure Lengend Snippet: Cell morphology of AoAF (A), HUVEC (B), and T/G HA-vSMC (C) was assessed on TCPS and on 13.7 kPa, 5.2 kPa, and 0.3 kPa PEG-based hydrogels.

Article Snippet: To yield hydrogels that support growth of human aortic adventitial fibroblasts (AoAFs; Lonza, Walkersville, MD), human aortic vascular smooth muscle cells (T/G HA-vSMCs; ATCC, Manassas, VA), and mouse fibroblast cells (NIH3T3; ATCC), bFGF (60 nM; Peprotech, Rocky Hill, NJ) was added during gel formation.

Techniques:

Journal: Journal of Biomedical Materials Research. Part a

Article Title: Differential effects of substrate modulus on human vascular endothelial, smooth muscle, and fibroblastic cells

doi: 10.1002/jbm.a.34075

Figure Lengend Snippet: Cell Titer Blue results for NIH3T3, AoAF, HUVEC, and T/G HA-vSMCs grown on heparinized PEG-based hydrogels containing fibronectin and growth factor. Mean fluorescence units (+ std dev; n = 4) are given to indicate number of viable cells attached during the first two hours of culture (A) and the change in cell number over time (B). Asterisks indicate statistically significant differences by One Way ANOVA (p<0.01).

Article Snippet: To yield hydrogels that support growth of human aortic adventitial fibroblasts (AoAFs; Lonza, Walkersville, MD), human aortic vascular smooth muscle cells (T/G HA-vSMCs; ATCC, Manassas, VA), and mouse fibroblast cells (NIH3T3; ATCC), bFGF (60 nM; Peprotech, Rocky Hill, NJ) was added during gel formation.

Techniques: Fluorescence

Journal: Journal of Biomedical Materials Research. Part a

Article Title: Differential effects of substrate modulus on human vascular endothelial, smooth muscle, and fibroblastic cells

doi: 10.1002/jbm.a.34075

Figure Lengend Snippet: Hierarchical cluster analysis of gene expression data. Genes whose expression was significantly different on hydrogels (low modulus = 0.3 kPa, medium modulus = 5.2 kPa, high modulus = 13.7 kPa) versus TCPS are shown for AoAF (A), HUVEC (B), and T/G HA-vSMCs (C) cells in heat maps. Columns represent each sample and rows represent each gene. Red and green in cells reflect high and low expression levels, respectively, as indicated in the scale bar (log2-transformed scale).

Article Snippet: To yield hydrogels that support growth of human aortic adventitial fibroblasts (AoAFs; Lonza, Walkersville, MD), human aortic vascular smooth muscle cells (T/G HA-vSMCs; ATCC, Manassas, VA), and mouse fibroblast cells (NIH3T3; ATCC), bFGF (60 nM; Peprotech, Rocky Hill, NJ) was added during gel formation.

Techniques: Gene Expression, Expressing, Transformation Assay